RNA Preparation and Purification
MyBioSource RNA (For fixed/embedded tissues)(without Phenol & Chloroform, need not DNase I digestion, directly use in RT-PCR or qPCR) Isolation Kit, 50 Tests
This kit is designed to quickly extract total RNA from formalin-fixed and paraffin-embedded tissue samples. The unique lysis solution/proteinase K quickly lyses cells to release RNA, and then the lysis mixture passes through a genomic DNA removal column, where genomic DNA is removed and RNA is filtered through. After the filtered RNA is adjusted with ethanol to adjust the binding conditions, the RNA is selectively adsorbed on the silicon matrix membrane in the spin column in a high-isolated salt state, and then through a series of rapid rinsing-centrifugation steps, the protein-removing solution and the rinsing solution remove the cells. Metabolites, proteins and other impurities are removed, and finally the low-salt RNase free H20 will elute the pure RNA from the silicon matrix membrane. The obtained RNA can be used in experiments such as reverse transcription PCR and fluorescent quantitative PCR.
Revvity Health Sciences Inc RNA Pico Sensitivity Assay Reagent Kit
RNA Pico Sensitivity Assay Reagent Kit
Macherey-Nagel NUCLEOSPIN 96 RNA BLD
Isolation of RNA from whole blood in proven 96‑well plate format. Selling Unit:192, Application:Isolation of RNA, MACHEREY NAGEL INC, mfg# 740225.2
New England Biolabs, Inc. Monarch® Buffer BX - 80 ml
The Monarch Buffer BX, a component of Monarch Nucleic Acid Purification Kits, is a guanidine-based buffer designed for binding steps in nucleic acid purification.
- A component of the Monarch RNA Cleanup Kits
- A guanidine-based buffer designed for binding steps in nucleic acid purification
- Monarch kit components are also available separately for added convenience
- Monarch kit components are also available separately for added convenience
New England Biolabs, Inc. NEBNext rRNA Depletion Kit (Bacteria) with RNA Sample Purification Beads – 96 reactions
The NEBNext rRNA Depletion Kit (Bacteria) employs the NEBNext RNase H-based RNA depletion workflow to target removal of rRNA from gram-positive and gram-negative organisms. The method is effective with intact and degraded RNA, from monocultures or samples with mixed bacterial species (e.g., metatranscriptomic). For convenience, this kit includes RNA Clean beads.
Research Products International Corp Zymo Direct-zol-96 RNA with TRI Reagent, 4 x 96 Preps
The Direct-zol-96 RNA Kits are RNA purification kits that provide a streamlined method for the purification of up to 10 µg (per well) of high-quality RNA directly from samples in TRI Reagent. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product. The extraction method inactivates viruses and other infectious agents. The procedure is easy: simply apply a sample in TRI Reagent® to the Zymo-Spin I-96 Plate, then bind, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc. The entire procedure typically takes about 30 minutes (per 2 plates).
New England Biolabs, Inc. NEBNext rRNA Depletion Kit v2 (Human, Mouse, Rat) with RNA Sample Purification Beads – 96 reactions
The NEBNext rRNA Depletion Kit (Human/Mouse/Rat) with RNA Sample Purification Beads employs an RNaseH-based method to deplete both cytoplasmic (5S rRNA, 5.8S rRNA, 18S rRNA and 28S rRNA) and mitochondrial ribosomal RNA (12S rRNA and 16S rRNA) from human, mouse and rat total RNA preparations. This product is suitable for both intact and degraded RNA (e.g., FFPE RNA). The resulting rRNA-depleted RNA is suitable for RNA-Seq, random-primed cDNA synthesis, or other downstream RNA analysis applications. For added convenience, this kit contains NEBNext RNA Sample Purification Beads (Agencourt RNAClean XP beads from Beckman Coulter).
Zyagen Labs Rat Liver Genomic DNA, 0.1mg
Rat Liver genomic DNA is a highly pure intact high molecular size DNA. It is extracted from single tissue of single donor and treated with DNase-free RNase to remove contaminant RNA. Genomic DNA is precisely measured by Nanodrop (a spectrophotometer technology) and stored at -80oCApplications:PCR Amplification, Southern blot analysis, genomic DNA methylation, single-nucleotide polymorphism (SNP) Analysis, profiling studies in gene expression, and DNA library construction
Zymo Research Corporation Quick-RNA 96™ Kit (2 x 96 Preps)
The Quick-RNA 96 Kit is designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 106) and tissue samples (up to 5 mg). The procedure combines a unique buffer system with Zymo-Spin™ plate technology to yield high quality total RNA (including small RNAs ~17-200 nt) in about 30 minutes. The procedure is simple: Add the provided RNA Lysis Buffer to a sample, then purify the RNA using the provided Silicon-A Plate. The result is highly-concentrated, DNA-free RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, sequencing etc.
Zymo Research Corporation ZR-96 RNA Clean & Concentrator™ Kit (2 x 96 Preps)
The kit allows for efficient RNA clean-up with the supplied Zymo-Spin™ I96 Plate. RNA is washed, then eluted and concentrated into 10 l of DNase/RNase-free Water. The highly concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.